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Abstract As a part of the Deciphering the Interplay between the Interstellar medium, Stars, and the Circumgalactic medium (DIISC) survey, we investigate indirect evidence of gas inflow into the disk of the galaxyNGC 99. We combine optical spectra from the Binospec spectrograph on the MMT telescope with optical imaging data from the Vatican Advanced Technology Telescope, radio Hi21 cm emission images from the NSF Karl G. Jansky’s Very Large Array, and UV spectroscopy from the Cosmic Origins Spectrograph on the Hubble Space Telescope. We measure emission lines (Hα, Hβ, [Oiii]λ5007, [Nii]λ6583, and [Sii]λ6717, 31) in 26 Hiiregions scattered about the galaxy and estimate a radial metallicity gradient of −0.017 dex kpc⁻¹using the N2 metallicity indicator. Two regions in the sample exhibit an anomalously low metallicity (ALM) of 12 + log(O/H) = 8.36 dex, which is ∼0.16 dex lower than other regions at that galactocentric radius. They also show a high difference between their Hiand Hαline of sight velocities on the order of 35 km s⁻¹. Chemical evolution modeling indicates gas accretion as the cause of the ALM regions. We find evidence for corotation between the interstellar medium ofNGC 99and Lyαclouds in its circumgalactic medium, which suggests a possible pathway for low metallicity gas accretion. We also calculate the resolved Fundamental Metallicity Relation (rFMR) on subkiloparsec scales using localized gas-phase metallicity, stellar mass surface density, and star formation rate surface density. The rFMR shows a similar trend as that found by previous localized and global FMR relations.